Molecular techniques for clinical diagnostic virology

Molecular techniques for clinical diagnostic virology.

A decade after the first studies were performed it is justifiable to claim that molecular techniques have revolutionised the work of the clinical virology laboratory. Hitherto, the role of the virology laboratory was often a retrospective diagnosis based on virus isolation and serology. Nevertheless, the epidemiological data collected in this way justified the continued activity of clinical vir...

Clinical Molecular Diagnostic Techniques: A Brief Review

L.-J.C. Wong (ed.), Next Generation Sequencing: Translation to Clinical Diagnostics, DOI 10.1007/978-1-4614-7001-4_2, © Springer Science+Business Media New York 2013 Abstract The identifi cation and characterization of the genetic basis of disease is often fundamental to diagnosis. Detection of pathogenic mutations in a DNA sample can lead to a diagnosis, possible prognosis, and prospective the...

COVID-19; History, Taxonomy, and Diagnostic Molecular and Immunological Techniques

In recent years, viral epidemics such as the coronavirus disease (COVID-19) have spread, and this outbreak is thought to be the result of animal-to-human transmission. Hence, accurate diagnostic tests to detect COVID-19 and antiviral antibodies in infected individuals are of utmost importance. This report describes the structure, history, taxonomy, and molecular and immunological techniques for...

Verification and validation of diagnostic laboratory tests in clinical virology.

This review summarizes major issues of verification and validation procedures and describes minimum requirements for verification and validation of diagnostic assays in clinical virology including instructions for CE/IVD-labeled as well as for self-developed ("home-brewed") tests or test systems. It covers techniques useful for detection of virus specific antibodies, for detection of viral anti...

Diagnostic molecular pathology: current techniques and clinical applications, part I.